Cárdenas, AnaAnaCárdenasJosé Moya‐DíazLucas BayonésMauricio MontenegroHenner KochAtsushi DoiFernando D. Marengo2025-08-252025-08-252019-11-2610.1111/apha.134172-s2.0-85076429573https://cris-uv-2.scimago.es/handle/123456789/3384WOS:000502031500001Abstract Aim It is widely accepted that the exocytosis of synaptic and secretory vesicles is triggered by Ca 2+ entry through voltage‐dependent Ca 2+ channels. However, there is evidence of an alternative mode of exocytosis induced by membrane depolarization but lacking Ca 2+ current and intracellular Ca 2+ increase. In this work we investigated if such a mechanism contributes to secretory vesicle exocytosis in mouse chromaffin cells. Methods Exocytosis was evaluated by patch‐clamp membrane capacitance measurements, carbon fibre amperometry and TIRF. Cytosolic Ca 2+ was estimated using epifluorescence microscopy and fluo‐8 (salt form). Results Cells stimulated by brief depolatizations in absence of extracellular Ca +2 show moderate but consistent exocytosis, even in presence of high cytosolic BAPTA concentration and pharmacological inhibition of Ca +2 release from intracellular stores. This exocytosis is tightly dependent on membrane potential, is inhibited by neurotoxin Bont‐B (cleaves the v‐SNARE synaptobrevin), is very fast (saturates with time constant <10 ms), it is followed by a fast endocytosis sensitive to the application of an anti‐dynamin monoclonal antibody, and recovers after depletion in <5 s. Finally, this exocytosis was inhibited by: (i) ω‐agatoxin IVA (blocks P/Q‐type Ca 2+ channel gating), (ii) in cells from knock‐out P/Q‐type Ca 2+ channel mice, and (iii) transfection of free synprint peptide (interferes in P/Q channel‐exocytic proteins association). Conclusion We demonstrated that Ca 2+ ‐independent and voltage‐dependent exocytosis is present in chromaffin cells. This process is tightly coupled to membrane depolarization, and is able to support secretion during action potentials at low basal rates. P/Q‐type Ca 2+ channels can operate as voltage sensors of this process.enacceso restringidoPhysiologyarticle