Nathalie Casanova-MoralesNataniel L. FigueroaKarol AlfaroFelipe MontenegroNelson P. BarreraJ. R. MazeChristian A. M. WilsonConejeros, PabloPabloConejerosEtienne Dague2025-12-082025-12-082019-11-0710.1371/journal.pone.02224682-s2.0-85074658811https://cris-uv-2.scimago.es/handle/123456789/8212WOS:000532694400008Optical tweezers have enabled the exploration of picoNewton forces and dynamics in single-molecule systems such as DNA and molecular motors. In this work, we used optical tweezers to study the folding/unfolding dynamics of the APTSTX1-aptamer, a single-stranded DNA molecule with high affinity for saxitoxin (STX), a lethal neurotoxin. By measuring the transition force during (un)folding processes, we were able to characterize and distinguish the conformational changes of this aptamer in the presence of magnesium ions and toxin. This work was supported by molecular dynamics (MD) simulations to propose an unfolding mechanism of the aptamer-Mg+2 complex. Our results are a step towards the development of new aptamer-based STX sensors that are potentially cheaper and more sensitive than current alternatives.enacceso abiertoAgricultural And Biological SciencesBiochemistry, Genetics And Molecular BiologyMultidisciplinary SciencesMedicinearticle