Emerson M. CarmonaMiguel FernandezJuan J. Alvear-AriasNeely, AlanAlanNeelyH. Peter LarssonOsvaldo AlvarezJose Antonio GarateLatorre, RamónRamónLatorreCarlos Gonzalez2025-12-072025-12-072021-05-0310.1073/pnas.20255561182-s2.0-85105314934https://cris-uv-2.scimago.es/handle/123456789/7398WOS:000651331900019Significance Acid extrusion in various types of cells and the reactive oxygen species production by the NADPH oxidase in phagocytes and microglia are mediated by the voltage-gated proton channel (H v 1). One of the biophysical properties essential for the H v 1 function is its ΔpH-dependent gating. Here, we measured the H v 1 gating currents’ pH dependence in the monomeric nonconducting D160N Ciona intestinalis H v 1 mutant. We demonstrated that H v 1 ΔpH-dependent gating is a consequence of voltage sensor modulation by direct gating currents recordings. Around 60% of the energy stored in the ΔpH is coupled to the H v 1 voltage sensor activation. Therefore, the proton chemical energy is transduced to the voltage sensor activation in H v 1 to produce ΔpH-dependent gating.enacceso abiertoMultidisciplinary SciencesMultidisciplinaryarticle